Description
This Human C-Type Lectin Domain Family 4 Member C (CLEC4C) ELISA Kit is intended for quantitative detection of human CLEC4C in cell culture supernates, serum and plasma (heparin, EDTA, citrate). Strip well format. Reagents for up to 96 tests.
This human CLEC4C ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CLEC4C has been precoated onto 96-well plates. Standards (Expression system for standard:, Immunogen sequence:) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CLEC4C is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CLEC4C amount of sample captured in plate.
The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated polyclonal antibody from goat